Biomedical subjects
Publications and source records attributed to A Arellano.
The technique and results of a new scalp flap that solves frontal baldness are described. As a bipediculate flap it offers a great amount of hair when advanced and has a low rate of complications. This kind of flap has been used by us for four years in 36 patients in the age group between 20 and 60 years. The results obtained with the flap have been very satisfactory.
This paper describes the isolation, purification and full characterization of the protamine from Sardinops sagax. The protein was purified by both gel filtration and ion exchange chromatography and its amino acid composition, electrophoretic behaviour and sequence were determined. We postulate that the protamine exhibits microheterogeneity with a major and a minor component. The molecular weight for the major component is 4377, as calculated from the sequence. The N-terminal amino acid is proline and the C-terminal is arginine. The amino acid composition is: Thr-Ser-Glx-(Pro)2-Gly-(Ala)3-(Val)2-(Lys)2-(Arg)20 The secondary structure for the protamines was also predicted and a revision of our previously published results in this area is discussed. We have called this protamine Sagaxine and an overall comparison with the hitherto described fish protamines is also made.
The results of grafts of fat obtained through liposuction are presented. They are the outcome of the current revitalization of the old technique of fat extraction and transplant. Both doctor and patient should be aware that a successful treatment is achieved through continuity. Fat grafts evolve with respect to time and permanence with reabsorption a part of the normal evolutionary process. Fat reinjection is a consequence of tissue reabsorption or a complement of the preceding injection. There is no complication associated with repeated reinjection. All cases reported here include patients with two or more reinjections, depending on their pathology and evolution.
Conjugates of fluorescein isothiocyanate with clupein YI, YII and Z, the protamines from Clupea palasii, were prepared and their fluorescence utilized to determine the rotational relaxation times of the proteins. All conjugates exhibited single component lifetimes near 4.05 ns. Linear isothermal Perrin plots were obtained for all conjugates; these data indicated rotational relaxation times of 3.33 ns for clupein YI and YII and 3.19 ns for clupein Z. These results and the results from our previous studies lead us to postulate globular conformations for the three proteins with hydrated molecular diameters of 22 A. Based on these findings a three dimensional model for Clupein YII is proposed.
This paper present the method and technical description of the fat graft injection with the material obtained from the same patient (donor area) to correct the affected area (receptor), and also have the finality to remark the good aesthetic results and the patient acceptance. A comparative of the procedure advantage with other filling materials is effected, providing the highly permanence and minimal complications. An evaluation since 1984 is made, referring the innocuousness and facility of the method. We employ the autogenous tissue to make the aesthetic correction of the face wrinkles, nasolabial folds, and depression. With this technique we improve defects like facial atrophy, radiodystrophies, acne, scars, etc. In the same way we use the fat injection in combined procedures with lipoplasty, blepharoplasty, rhinoplasty and facelift. We made this histological study taking postoperative biopsies in some patients.
Seventy patients with stage IV head and neck cancer were treated by two courses of induction chemotherapy followed by surgery plus postoperative irradiation (7) or radical radiotherapy (62). One patient renounced to further treatment after chemotherapy. Each chemotherapy course consisted of methotrexate (40 mg/m2 days 1 and 14), bleomycin (10 mg/m2 days 1, 7 and 14), and cisplatin (50 mg/m2 day 4). Three patients did not complete the two courses of chemotherapy planned due to bad tolerance whereas the rest of the patients tolerated chemotherapy well. 2 CR (3%) and 35 PR (50%) were achieved for an overall response rate to chemotherapy of 53%. 35 (50%) CR were achieved with the whole treatment schedule. The 5-year disease-free survival is 26% for the whole group of patients. The prior response to chemotherapy neither influenced the complete responses to treatment nor the relapse-free survival. The addition of this chemotherapy to conventional treatment was of no value.
Fifty-seven patients with T1 transitional cell carcinoma of the bladder, 57 patients with T2-3 and 10 patients with T4 were treated by radical irradiation +/- salvage cystectomy. Thirty-five patients with T1 were treated by transurethral resection (TUR) plus local chemotherapy +/- salvage cystectomy. Eleven patients with T2-3 were treated with 5 X 4 Gy in one week followed by cystectomy while four patients with T2-3 were treated with 40 Gy in 4 weeks also followed by cystectomy. Five patients with T4 were treated with 40 Gy in 4 weeks plus cystectomy. Seven year survival rates are: 69% for T1 treated by radical irradiation, 43% for T1 treated by TUR (p less than 0.02), 36% for T2-3 treated either by radical irradiation or 5 X 4 Gy plus cystectomy, and 18% for T4 treated by radical irradiation. All the survival rates indicated are free of disease and include the cases in which salvage cystectomy was successfully performed. Sixty per cent of the cured patients conserved very good functional bladders. We believe that external beam irradiation plus salvage cystectomy is indicated in the treatment of T1 and T2-3 tumours of the bladder.
After giving an introduction on the surgical treatment of alopecia in the last years, we talk about our experience with more than 2,500 patients. We discussed in individual chapters each one of the four major methods for the surgical management of male pattern alopecia: punch grafts, strip grafts, scalp reduction, flaps and combined techniques. In general more than the half of those patients have been seen and controlled for long time, because the most of them have continued with the clinic treatment in correspondence with the skin-hair pathology.