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Biomedical subjects

A Anthony

Publications and source records attributed to A Anthony.

119 records · Page 7Linked to original sources
PubMed1976

Quantitative cytophotometry evaluated as a method for analyses of herpes simplex viral deoxyribonucleic acid synthesis.

Feulgen deoxyribonucleic acid (F-DNA) microspectrophotometry was evaluated as a potential tool for quantification of herpes simplex virus type 1 (HSV-1) and 2(HSV-2) DNA synthesis in single cells. Since HSV DNA synthesis has been extensively studied using incorporation of radioactive precursors into viral and cellular DNA, microspectrophotometric measures were correlated with biochemical data obtained using tritium-labeled thymidine ([3H]TdR). It was established that: (i) viral-induced increaae in F-DNA can be cytophotometrically detected between 1 and 6 h postinjection (p.i.), which corresponds to the initial incorporation of [3H) TdR into viral DNA; (ii) peak F-DNA levels occurred 8 h p.i., which supported cytological observations of a more rapid development of an inclusion body in HSV-2-infected nuclei. Despite the fact that F-DNA cytophotometry is unable to distinguish between cell and viral DNA, the overall study supports the existence of a good correlation between data obtained using microspectrophotometric and conventional isotope methods. Furthermore, cytophotometry complements biochemical evaluations in that it permits analyses of DNA changes on a single-cell basis or the detection of infection in very small numbers of cells.

Cell Nucleus↗
PubMed1975

Differential feulgen-deoxyribonucleic acid hydrolysis patterns of Herpes simplex virus type 1 and type 2 infected cells.

Infection of human embryonic lung cells with herpes simplex virus type 1 (HSV-1) and herpes simplex type 1 (HSV-2) resulted in: (a) qualitative (nuclear cytopathologic) alterations and quantitative (nuclear area) differences in infected compared to control nuclei; (b) increased Feulgen-deoxyribonucleic acid (F-DNA) amounts in infected cells, probably due to viral DNA; (c) higher F-DNA levels in HSV-2 infected cells; and (d) increased rates of F-DNA hydrolysis in viral-infected as compared to uninfected nuclei.

Cell Line↗
PubMedDate not supplied

Pediatric pain patterns during Ilizarov treatment of limb length discrepancy and angular deformity.

The Ilizarov method of gradual incremental distraction of long-bone deformities has provided dramatic functional improvement in children, but its cost in terms of pain may be high. The intensity of pain experienced and its progression with time was measured prospectively in 23 patients (age 11-20 years) by the Adolescent and Pediatric Pain Tool (APPT) and the Hospital for Sick Children (HSC) Pain Scale at four test intervals. These instruments were composed of visual analogue scales, body diagrams, and word lists. Results indicated high levels of pain extending over several months, in sharp contrast to the general orthopedic pain experience. The prolongation of pain throughout the entire correction and consolidation processes must be recognized to ensure appropriate management, particularly in the outpatient setting.

Adolescent↗