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Biomedical subjects

A Angulo

Publications and source records attributed to A Angulo.

43 records · Page 3Linked to original sources

Retroperitoneal sacrocolpopexy for correction of prolapse of vaginal vault.

The problem of prolapse of vaginal vault has a clearly defined anatomic basis and so does it surgical restoration. Herein, we present our experience with a technique that is a modification of the abdominal sacrocolpopexy of Randall and Nichols. Our procedure is characterized by the use of a segment of Teflon (polytetrafluoroethylene) vascular prosthesis placed in the retroperitoneum at the sacral concavity to fasten the vaginal vault to the promontory. We have obtained excellent results with its application in 18 patients, who had a return to normal sexual activity and no recurrences during a follow-up period of 36 months.

Abdominal Muscles↗

The occurrence of salmonellae in kitchens of care institutions.

Sporadic cases of salmonellosis always by the same serotypes occurred in two different care institutions. In the first institution the cases had been caused by S. binza and S. eimsbuettel, in the second by S. mons and S. braenderup. The cases of salmonellosis prompted studies into the occurrence of these Salmonella serotypes in the kitchens of the relevant institutions, as well as into the occurrence of salmonellas in kitchens of care institutions in general. Using Moore's swabs the relevant Salmonella serotypes could be demonstrated in the kitchens of the institutions with sporadic cases of salmonellosis, while in kitchens of 15 other arbitrary chosen institutions Salmonella could not be detected at all. This demonstrated that the occurrence of sporadic cases of salmonellosis was related to the occurrence of salmonellas in the kitchens. Then investigations were started to establish the origin of the salmonellas. There was a mice plague in one institution. Mouse-dung was found positive for the relevant Salmonella serotype(s). Cases of salmonellas did not occur any more when the mice had been eradicated. It was concluded that the sporadic cases of salmonellosis in that institution were strongly associated with the presence of mice. In the other institution it appeared that disinfection was inadequate and that salmonellae were spread all over the kitchen environment during cleaning, leading to recontamination. After thorough disinfection and adaptation of cleaning and disinfection procedures sporadic cases of salmonellosis did not occur anymore. Results of these investigations demonstrated the important role of vermin and proper cleaning and disinfection in food hygiene. Moreover, the sporadic cases of salmonellosis came to light because gastro-enteritis was a reason for examination of faecal specimens of patients. This is not usual in many care institutions. However, in this way problems in hygiene can be traced, especially in the kitchen. For this reason it is recommended strongly to examine faecal specimens in cases of gastro-enteritis, even in sporadic cases.

Accident Prevention↗

A Golgi study on the nucleus accumbens septi of the rat.

Golgi impregnations of one-month-old Wistar rats show 3 different neuronal types in the nucleus accumbens septi. These types could be clearly established on the basis of the size and shape of the soma and dendritic pattern; dendritic spines could not be used as a useful criterion for distinguishing among the 3 types. The type I neurons were the most widely distributed, while the giant type II cells are restricted to the ventral region. Type III neurons are fusiform cells; according to the axon branching, these neurons and those of type I may be interneurons.

Animals↗

Assessing conservation biology priorities through the development of biodiversity indicators.

Biodiversity erosion is now a recognized phenomenon within the scientific community, and it is understood that there is an urgent need for action to relieve this loss. However, no consensus exists on how to go about achieving this goal. This is due to two kinds of problems: namely, that there are many different reasons which can motivate conservation attempts and hence they can lead to different priorities, sometimes in direct contrast with one another; and that of the complexity of the natural world which we have to operate in. Regarding the latter, several authors have lamented a severe lack of theoretical support for many conservation decisions. We briefly review some of the criteria that are commonly adopted in conservation, and indicate some of their problems; we then introduce a new theoretical framework based on the use of a series of biological indicators, many of which have already been proposed in the past but never fully explained and/or adopted. In addition, we propose two indices for one of these potential indicators, i.e. evolutionary potential. We conclude with an appeal for a greater collaboration between conservation scientists and evolutionary biologists, in order to encompass evolutionary evidence in the decision-making process. In doing so, we hope to increase the chances of successful conservation efforts.

Animals↗

Progressive hepatocytic fatty infiltration in rats with prehepatic portal hypertension.

BACKGROUND/AIMS: Homogenous evolution, with a narrow range of portal hypertension, degree of portosystemic shunt and hepatic atrophy has been described in the experimental model of prehepatic portal hypertension in the rat. However the great differences observed in the rats' liver weight could be attributed to a pathological alteration of the liver. Based on this, we performed an evolutive histological study of the liver. This study shows the existence of a progressive hepatocytic fatty infiltration. METHODOLOGY: Male Wistar rats with portal hypertension induced by triple stenosing ligation of the portal vein at 1 month (group II, n=4) and at 1 year (group IV, n=10) of postoperative evolution were used. The portal pressure, body, liver and splenic weights, types of collateral circulation and degree of mesenteric venous congestion were studied. The intracytoplasmatic lipid microvacuoles were quantified in hepatocytes with an image analyzer (software MIP/CID, Spain). The results were compared with those obtained in control rats with the same evolutive periods (Groups I and III). RESULTS: The hepatic fatty infiltration in Group II (TPVS 1 month) (30.12+/-53.92 micron2) is similar to that presented by Group III (Control 1 year) (16.52+/-45.20 micron2), while there is an increase (p<0.001) in Group IV (triple portal vein stenosis 1 year) (182.03+/-371.42 micron2) in relation to the other groups studied. The progressive hepatic fatty infiltration in triple portal vein stenosis rats is associated with a decrease of portal pressure and of the incidence of liver hepatic atrophy, portosystemic collateral circulation and mesenteric venous congestion. CONCLUSIONS: TPVS produces progressive hepatocytic fatty infiltration in the rat so that this prehepatic portal hypertension experimental model could also be considered as a hepatic steatosis model.

Animals↗