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Biomedical subjects

A Andersson

Publications and source records attributed to A Andersson.

439 records · Page 25Linked to original sources
PubMedDate not supplied

[3H]paroxetine binding in human platelets in relation to age and sex.

The binding of [3H]paroxetine to serotonin uptake sites in human platelets from 47 individuals between 15 and 95 years of age was investigated. There were no significant age-related changes in maximal binding capacity (Bmax) or apparent binding affinity (Kd). There were no significant differences in binding between males (n = 19) and females (n = 28).

Adolescent↗
PubMedDate not supplied

Platelet [3H]paroxetine binding to 5-HT uptake sites in Alzheimer's disease.

Platelet serotonin (5-hydroxytryptamine, 5-HT) uptake sites were studied in a control group (n = 30) and an Alzheimer group (n = 40) using [3H]paroxetine as radioligand. The maximum number of binding sites (Bmax) for control (1250 +/- 60 fmol/mg protein) was not different from the Alzheimer group (1280 +/- 40 fmol/mg protein). There were no differences in apparent binding affinity (Kd): 0.046 (0.024-0.062) nM for control and 0.040 (0.027-0.061) nM for Alzheimer. Thus even though several previous studies have demonstrated marked atrophy of 5-HT containing neurites and 5-HT uptake sites in Alzheimer's disease, these findings are not found in the periphery on platelets. The platelet 5-HT uptake site cannot be used as a peripheral marker of Alzheimer's disease.

Aged↗
PubMedDate not supplied

Experimental pancreatic transplantation in diabetes.

Although transplantations of vascularized pancreas in diabetic patients show steadily improving results, the immediate operative risks and life-long immunosuppressive medication involved represent considerable disadvantages. Efforts are being made to develop simpler and safer methods of transplantation with isolated pancreatic islet grafts, e.g., isolated islets, fetal pancreas, or dispersed adult pancreas. Iso-, allo-, and xenografts of such preparations have been shown to reverse diabetes in animals. However, attempts to apply these techniques in clinical practice have remained largely unsuccessful, and major technical advances are needed before success is achieved. Attempts to use whole, segmented, or isolated islets from pancreatic grafts as a cure for diabetes in animals and in diabetic patients are reviewed. The importance to the graft's permanent function, of adequate preparation and storage of the graft, and of beta-cell growth and vascularization are reviewed. Various forms of immunomodulation by pretreatment of grafts in vitro have been employed in animal models of diabetes, but none of these have yet been employed with long-term success in humans. Recurrence of a specific autoimmune response toward the beta-cell in a spontaneously diabetic recipient is a potential mechanism for destruction of transplanted islet tissue.

Animals↗
PubMedDate not supplied

Phospholipid metabolism of A-cell enriched pancreatic islets.

Glucose exerts different effects on the hormone secretion from pancreatic B- and A-cells. In order to assess whether this can be linked to a difference in the effects of glucose on the phospholipid metabolism of these two cell types, islets were isolated from streptozotocin-treated (A-cell enriched islets) or normal guinea-pigs (B-cell rich islets). A-cell enriched islets contained more phospholipids when correction was made for the difference in size. Glucose-stimulated (16.7 mM) phospholipid and diacylglycerol biosynthesis, as estimated from 14C-glucose incorporation, was somewhat more stimulated in normal islets as compared with that of A-cell enriched islets. Triacylglycerol biosynthesis was, however, stimulated to the same extent. The pattern of 14C-glucose incorporation into the different phospholipid classes was very similar in both kinds of islets. Despite their smaller size, A-cell enriched islets incorporated, however, as much 14C-glucose as normal islets into most phospholipid classes. The present results are in line with previous findings of differences in glucose handling between the two cell types.

Animals↗