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Biomedical subjects

A Alonso

Publications and source records attributed to A Alonso.

At least 523 records · Page 29Linked to original sources
PubMed1979

[Communication between the right coronary artery and the right atrium. Apropos of 2 surgical cases].

Two cases of anomalous communication of the right coronary artery with the right atrium (the so-called coronary artery fistula) are presented. Both were young females in which a continuous murmur was heard during routine examination. The x-ray image was typical, with a huge bulge in the right border similar to that seen in cases of right atrium enlargement. The ECG was normal in both patients. The diagnosis was made during cardiac catheterization and angiocardiography. A slight left-to-right shunt was present and the aneurismatic dilatation of right coronary artery and communication with the right atrium could be documented. In both cases a surgical closure of the defect was performed, both being well after two years and eight months respectively.

Adult↗
PubMed1978

Apparent half-lives of sugar transport proteins in Saccharomyces cerevisiae.

Using incubation in the presence of 0.4 mM cycloheximide the half-lives of the principal membrane transport proteins in baker's yeast were found to be: more than 24 h for the constitutive glucose carrier, 2.2 h for the inducible galactose carrier, 1.2 h for the inducible maltose carrier and 0.8 h for the inducible alpha-methyl-D-glucoside carrier. The distinct nature of the two last-named carriers was thus supported. De-induction of the galactose carrier was enhanced in the presence of glucose plus cycloheximide but not of either substance alone. Chloramphenicol suppressed all effects of cycloheximide. In contrast to the enzymes of galactose metabolism, the induction of the glactose carrier was not under the control of a mitochondrial factor and took place in a rho-mutant. The system induced by maltose but not the one induced by alpha-methyl-D-glucoside was de-induced rapidly by the intervention of a cytoplasm-synthesized protein.

Carbohydrate Metabolism↗
PubMed1978

Cross-sex supervision for cross-sex therapy.

The female supervisor can help the male therapist in training to overcome problems in cross-sex therapy by presenting herself as a role model, providing relevant literature, and sharing her own experiences as a woman and as a professional. By enabling the male trainee to identify safely with a woman in a position of relative power, she can help him to deal with the intrinsic limitations of empathy and with other problems, such as "countertransference deafness," that may block his work with female patients.

Adult↗
PubMed1978

[Indications of vascular exploration in the diagnosis of abdominal neuroblastoma (author's transl)].

The criteria on the indications of angiography are shown in the diagnosis of abdominal neuroblastoma, stressing its' utility in situations where conventional procedures do not supply an accurate diagnosis. Angiography happens to be fundamental for diagnosis retroperitoneal tumours which eventually result in a silent kidney as well as for the differential diagnosis of some hepatic tumours and Pepper's syndrome.

Abdominal Neoplasms↗
PubMed1978

Carotenoid absorption in chicken intestine.

The powdered flowers of marigold (Tagetes erecta) are used as a cheap source of carotenoids in avicultura. Lutein (3,3'-dyhydroxi-alpha-carotene) constitutes up to 85 to 90% of marigold carotenoids. In the plant, lutein is found esterified to palmitic or estearic acid. In chicken, carotenoid is hydrolized in the first portion of the small intestine, and absorbed as free lutein. After the absorption, lutein is not re-esterified in the different chicken tissues.

Animals↗
PubMed1977

Indications for pneumoperitoneum in the diagnosis of congenital anomalies in the umbilical region.

Thirty pneumoperitoneograms were performed postmortem and 5 were performed for diagnosis: the technique itself, the radiological anatomy of the umbilical region, and the usefulness of the procedure in diagnosis are discussed. Pneumoperitoneum may be indicated in the investigation of a bleeding Meckel's diverticulum, in the exclusion or confirmation of remnants of the omphalomesenteric duct, in chronically moist lesions of the umbilicus resistant to symptomatic treatment, in suspected cases of non-communicating urachal cysts which cannot be diagnosed by cystogram, and in the differential diagnosis of abdominal tumours related to the umbilical region.

Abdominal Neoplasms↗
PubMed1977

Histone phosphorylation and synthesis of DNA and RNA during phases of proliferation and differentiation induced in mouse epidermis by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate.

The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induces discrete waves of proliferation and differentiation when applied to mouse epidermis. The aim of this study was to elucidate the temporal relationship between histone phosphorylation and synthesis of DNA and RNA in mouse epidermis during the process of tumor promotion. This investigation as facilitated by choosing a low but nevertheless strongly tumor-promoting dose of TPA (0.002 micronmole/mouse), which induced the epidermal cells to go through only one round of DNA synthesis and cell division. Histones were isolated from mouse epidermis, and the rates of phosphorylation of the individual histone species were determined at different times after treatment with TPA. The results demonstrated that the phosphorylation of H1 histone was initiated at about the same time as the synthesis of DNA but continued past the S phase and reached a maximum simultaneously with the maximum in the epidermal mitotic rate. The only other histone that phosphorylated to any significant extent was Histone H2A. From the results obtained, it was concluded that histone phosphorylation in the epidermis is related to the processes of DNA replication and mitosis after stimulation with TPA. Positive evidence for the activation of specific genes, which has been proposed by other authors to be important in the promotion of epidermal tumors, was not found in this investigation.

Animals↗
PubMed1976

Characterization of the RNA transcribed in vitro from native mammalian DNA by Escherichia coli RNA polymerase.

High-molecular-weight native mouse DNA was transcribed with Escherichia coli RNA polymerase under low salt conditions, and the nature of the DNA sequences transcribed determined by molecular hybridization. The results indicated that E. coli RNA polymerase does not transcribe the sequences in native mouse DNA randomly under these conditions. First, hybridization with a large excess of mouse DNA showed that no more than 5% of the RNA synthesized had been transcribed from repeated sequences in the DNA. Second, hybridization with tracer amounts of labelled non-repeated mouse DNA indicated that the bulk of the RNA had been transcribed from less than 1% of the non-repeated sequences and only about 10% had been transcribed from a further 25% of these sequences; the remaining non-repeated sequences in the DNA, amounting to 50% of the genome, were not represented in the RNA synthesized in vitro to any detectable extent. Third, the proportion (40%) of complementary DNA transcribed from mouse-liver nuclear polyadenylated RNA which hybridized with the RNA synthesized in vitro was significantly greater than would have been expected if transcription had been random. The data have also been interpreted as indicating the presence of two types of initiation site for E. coli RNA polymerase in the non-repeated sequences in mouse DNA. The frequencies of their occurrence have been calculated to be one per 150 000 base-pairs and one per 500 base-pairs, respectively.

Animals↗
PubMed1975

Solid-state protein kinase. A tool for post-synthetically modifying and radioactively labeling proteins in vitro.

The capacity of partially purified rat muscle protein kinase coupled to cyanogen-bromide-activated Sepharose 4B to (radio-)phosphorylate proteins in vitro was evaluated using histones from calf thymus and rat liver and certain proteins as substrates. Data are presented which point to a low substrate specificity of this enzyme. It is demonstrated that even within a short time period histones are efficiently phosphorylated without the introduction of contaminating (phospho-)proteins. Therebye phosphoserine residues are formed. The phosphorylation reaction usually performed at 30 degrees C is shown to function quite efficiently also at 4 degrees C. It proceeds even at 30 degrees C for several hours at pH values close to the physiological range without the release of proteins from the solid matrix. The phosphorus transfer can be largely increased with the use of high ATP concentrations. The stability of the substrates is sufficient to suggest a wide applicability of this solid-state protein kinase in the phosphorylation of proteins either for labeling or as a tool to modify proteins post-synthetically under gentle conditions. The solid enzyme seems to be suitable for radioactively labeling proteins of more complex biological structures, such as membrane surfaces.

Animals↗