Biomedical subjects
Publications and source records attributed to A Agrawal.
Entamoeba histolytica possesses significant acid phosphatase activity as compared to alkaline phosphatase activity. The acid phosphatase activity in the amoebic cells eluted at higher saline concentration as three distinct peaks at 200, 300 and 400 mM sodium chloride.
Fifty nodes of Hodgkin's disease, 50 of non-Hodgkin's lymphoma and 20 of reactive hyperplasia revealed varying degree of mast cell reactivity. It was maximum in reactive lymph nodes, sinuses and interfollicular areas being infiltrated more than zones. The number of mast cells was more in nodular sclerosis variety of Hodgkin's disease than other Rye-subtypes, and more in Hodgkin's group than non-Hodgkin's, out of the latter, the diffuse histiocytic lymphoma revealed higher number. Analysis of a larger series and follow-up of these patients are indicated to establish the possible reactionary nature of mast cell reactivity in lymphomas, and the prognostic bearing, if any.
We report a case of herpes zoster oticus with involvement of the mandibular division of the trigeminal nerve and loss of taste sensation in the anterior two third of the tongue. Infranuclear facial palsy and sensorineural deafness were also present.
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The action of phanquone on the incorporation of (14C)1-DL-valine and (14C)-8-adenine in TCA insoluble fractions of Entamoeba histolytica was examined as indices of RNA and protein synthesis respectively. The former was found to show greater sensitivity to the drug in terms of the effect manifested in the dose response curves for these parameters. Electron microscopic investigations with increasing concentrations of the drug demonstrated progressive vacuolization with concomitant dissolution of ribosomal helices. The drug had no significant effect on nucleus and plasma membrane even at concentration as high as 200 micrograms/ml.
Two hundred and forty seven pregnant women at term and their offsprings were studied. Maternal skinfold thickness was measured from ten different sites and newborn skinfold thickness was measured from four different sites in each set of cases. Maternal skinfold thickness was positively correlated with the birthweight and skinfold thickness of the offspring. The birthweight was also positively correlated with the skinfold thickness of offspring. The maternal axillary sites and offspring's subscapular site had higher inter correlation as well as with the birthweight of offspring.
The risks of viral transmission from trauma patients is a continuing concern to those involved in their care. However, the prevalence of hepatitis (HPT) in trauma patients is poorly described. The purpose of this study is to evaluate the prevalence of HPT in trauma patients admitted to an urban trauma center. Two hundred sixty-four consecutive admissions to an urban Level I trauma center underwent serologic screening for HPT. Risk factors were assessed by direct patient questioning. Serologic evidence of HPT B was found in 19.7 per cent of patients. Intravenous (IV) drug abusers represented eight per cent of the study population; this group had a 67 per cent rate of seropositivity. Hepatitis A was not found in any patient. Antigenemia was found in 1.9 per cent of patients. It is concluded that HPT B seropositivity is common in trauma patients. IV drug abusers have particularly high prevalence of HPT. This high prevalence rate of HPT B serology poses a significant risk to those involved with the care of trauma patients. The authors suggest that specific protocols to avoid the transmission of viral disease should be mandatory in urban trauma centers.
In the recent past, several herbal drugs have proved their efficacy in decreasing anxiety and tension. Until recently, limited scientific study has been done to prove the clinical importance of these herbal drugs. Geriforte is an indigenous compound commonly advocated for arresting the aging process. This remedy has also been found beneficial in reducing anxiety and stress.-34 diagnosed cases of anxiety neurosis and 24 apparently normal individuals were selected for the present study. After 12 weeks of drug therapy a significant reduction in 5-HT levels was observed. Circulating MAO and GABA showed an increasing trend along with decreased glutamic acid levels after treatment, in the neurotic anxiety cases. Psychological complaints considerably decreased after 12 weeks of therapy. Since Geriforte regulates the altered circulating biogenic amines, this remedy can be advocated as an adjunctive therapy in the management of stress disorders.
Axenically grown E. histolytica possess significant acid phosphatase activity. The Km of the enzyme was found to be 7.1 x 10(-3) and was maximally active at pH 4.5. Acid phosphatase activity was significantly inhibited by Cu2+, cysteine and was activated by tartrate and fluoride. It was found that E. histolytica acid phosphatase differs in some properties as compared to the enzyme reported from other sources.
Metronidazole (19 micrograms/ml) caused progressive increase in vacuolization in Entamoeba histolytica cells with disintegration of its plasma membrane leading to almost complete disappearance of the latter within 3 hours. The drug also induced formation of helical ribosomal aggregates in the cytoplasm and disappearance of button like structures inside the nucleus.
C-reactive protein (CRP) was found to be one of the major components of the haemolymph of A. fulica with a normal level of approximately 2 mg CRP/ml haemolymph. Immunological cross-reactivity was found between CRP from A. fulica, a mollusc and Limulus polyphemus, an arthropod. The present finding demonstrates the evolutionary significance of C-reactive protein.
In mercury-treated rats, C-reactive protein (CRP) has been found to be synthesized in the liver which, in turn, sequesters mercury resulting in the denaturation of the protein into subunits. The subunits retain the mercury and are released into the serum from where it gets excreted. Native CRP and CRP-subunits have been found to have different antigenicity. Thus one of the physiological roles of CRP seems to be detoxication of heavy metals.
A metallothionein-like protein (MLP) from the serum of mercury-treated rats was isolated while purifying an acute phase plasma protein, C-reactive protein, by its Ca2(+)-dependent affinity to phosphorylcholine (PC)-Sepharose column. The MLP was further purified by a single DEAE-Sephacel ion-exchange chromatography. The MLP is similar to mammalian hepatic Zn-metallothionein on the basis of its low molecular weight of approximately 7000, 7 g Zn atoms/molecule of MLP and an absorption maximum at 220 nm. The purity of the protein was confirmed by double immunodiffusion test against anti-MLP antiserum raised in a rabbit. Immunologically MLP was detected not only in the hepatic cytosol, serum and urine from Hg-treated rats but also in the serum and hepatic cytosol of untreated rats. Using PC-Sepharose column, MLP could not be purified from normal serum.
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The C-reactive proteins (CRP) from both rat and Limulus were found to bind mercury (Hg) in both in vivo and in vitro conditions. CRP has high-affinity binding sites for Hg as evidenced by the loss of free sulfhydryl groups, arrested mobility in polyacrylamide gel electrophoresis, and the consumption of CRP in the serum after Hg administration. The binding was tight as it could not be inhibited either by the addition of cysteine or EDTA. By using a direct titration method it was shown that binding of Hg to CRP was saturable at a molar ratio of Hg/CRP = 13.11. The possibility that CRP may act as a scavenger for Hg is discussed.
The action of antiamoebic drugs on the incorporation of (14C) 1-DL-valine and (14C)-8 adenine in TCA insoluble fraction of axenic Entamoeba histolytica was investigated. Adenine showed greater sensitivity to all the drugs tested viz. camoquin, chloroquine, diodoquin, metronidazole, entobex and diloxanide furoate except enterovioform.
The possibility of stimulation of C-reactive protein (CRP) due to inflammation and necrosis caused by cadmium was investigated. Cadmium injection (less than 3 mg/kg body weight) in white rat was found to be associated with the appearance of CRP in liver cytosol after 8 hr and in serum after 18 hr of injection. Although, no definite dose-response relationship was found, yet CRP level was elevated drastically, varying from 1-4 mg/g wet weight liver and 0.3-0.7 mg/ml serum. CRP-positive serum from Cd-treated rats precipitated with normal rat serum on agarose-covered slides in the presence of 1 mM CaCl2. Evidences for raised level of CRP indicates acute tissue damage in the animal.