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Biomedical subjects

A Adoutte

Publications and source records attributed to A Adoutte.

44 records · Page 3Linked to original sources
PubMed1975

Diaminobenzidine reactivity of mitochondria and peroxisomes in Tetrahymena and in wild-type and cytochrome oxidase-deficient Paramecium.

Reactivity of mitochondria and peroxisomes to diaminobenzidine was investigated in Tetrahymena pyriformis and in wild-type and cytochrome oxidase-deficient Paramecium aurelia. Wild-type and cytochrome oxidase-deficient Paramecium gave positive mitochondrial reactions in the absence of added H2O2, and the deposits were enhanced by the addition of H2O2, whereas Tetrahymena gave positive mitochondrial reactions only upon addition of H2O2. These results are discussed in the light of the current ideas concerning the mechanism of staining by diaminobenzidine. Peroxisome-like organelles which react positively to diaminobenzidine, the reaction being partially inhibited by aminotriazole, were identified in both protozoa.

3,3'-Diaminobenzidine↗
PubMed1974

Symposium No. 8: Non-chromosomal inheritance. Genetic control of mitochondria in paramecium.

Mitochondrial mutations for resistance to various antibiotics (erythromycin, chloramphenicol, spiramycin, mikamycin) have been obtained in Paramecium aurelia and their properties are reviewed. Using these mitochondrial markers, the interactions between nucleus and mitochondria have been studied in two ways: by microinjection of mitochondria from one stock or species into other stocks and species of P. aurelia and by a genetic study of a nuclear mutation affecting mitochondrial multiplication. Both types of experiments show: (1) that there may exist incompatibility between a given type of mitochondria and the cell into which they are introduced and (2) that through multiplication in the host cell, mitochondrial properties can be modified. The possible basis for incompatibility and host-induced modifications is discussed.

Anti-Bacterial Agents↗
PubMed1972

The effects of erythromycin and chloramphenicol on the ultrastructure of mitochondria in sensitive and resistant strains of Paramecium.

The effects on cell structure of 12 hr to 6 days of exposure to erythromycin or chloramphenicol, two antibiotics known to inhibit specifically the mitochondrial protein synthesizing system, have been studied in the ciliate Paramecium aurelia. A wild type strain (sensitive to both antibiotics) and three mutant strains carrying cytoplasmically inherited mutations conferring resistance to one or the other antibiotic have been used In sensitive cells both antibiotics lead to a progressive and profound alteration of mitochondrial structure evidenced by an elongation of the organelle, a considerable decrease in the number of cristae, and the appearance of some abnormal lamellar cristae and of rigid plates of periodic structure. The modifications of cell structure, then, are mainly restricted to mitochondrial cristae. The three resistant mutants studied, on the contrary, retain normal or nearly normal mitochondrial structure in the presence of the antibiotic to which they are resistant. This fact is in good agreement with the postulated location in the mitochondrial DNA of the resistance mutations studied. The results are discussed in the light of present knowledge concerning the function of the mitochondrial protein-synthesizing system.

Animals↗