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Biomedical subjects

A A Mustafa

Publications and source records attributed to A A Mustafa.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Effect of venom from Cerastes cerastes (Egyptian sand viper) on luminol-dependent chemiluminescence of human blood phagocyte cells.

Cerastes cerastes venom added in vitro to human whole blood caused a marked inhibitory effect on the luminol-dependent chemiluminescence induced by phorbol myristate acetate (PMA) or opsonized zymosan on phagocyte cells. The inhibitory effect produced by the venom was both dose- and time-dependent when PMA was used as the stimulant of the oxidative burst. Similarly, the venom produced a significant inhibitory effect when added to isolated polymorphonuclear leukocytes (PMNs). Incubation of the isolated PMNs with the highest concentration of the venom used (1000 micrograms/ml), however, did not result in any significant disruption of the membranes of these cells. The effect of the venom on the isolated PMNs was also reversible following washing of the venom-treated cells with phosphate-buffered saline. The scavenger of reactive oxygen species such as superoxide dismutase, catalase and dimethyl sulphoxide potentiated the effect of the venom on the luminol-dependent chemiluminescence of human phagocyte cells. Sodium azide, the myeloperoxidase inhibitor, and sodium benzoate produced a similar potentiating effect. The results suggest that some of the toxicity of the venom may be mediated by an action on the phagocytic immune system.

Animals↗
PubMedDate not supplied

A densitometric method for quantitative analysis of the left ventricle performance using i.v. digital subtraction angiography.

In this paper we present a method we used to provide quantitative description of the systolic and diastolic temporal function of the left ventricle (LV). Additional parameters, such as peak filling and ejection rates, times to end systole and end diastole, and temporal changes in slow and fast filling are obtained. The volumes associated with these parameters are also calculated. Correlation between LV volume changes during the cardiac style and corresponding "density" variations was confirmed. Time-density curves were obtained from selected cardiac cycles in each study. We used the polynomial fitting technique to fit the time density curves and developed a computer algorithm for deriving the relevant parameters. Data from a total of 18 patients with ischemic heart or valvular diseases, who underwent I.V. ventriculography was analysed using our method. Some of these patients were forwarded for repeated digital subtraction angiography (DSA) examination before and after intervention therapy to evaluate the effectiveness of treatment. In comparison to the geometric method for the analysis of LV performance, our method is generally faster and simpler to employ. The method was effective in detecting variations in the peak ejection and filling rates in our group of patients before and after interventional therapy.

Absorptiometry, Photon↗